Natural therapeutic factors are widely used as an important adjuvant therapy in various cardiovascular andNatural therapeutic factors are widely used as an important adjuvant therapy in various cardiovascular andcerebrovascular disorders. The aim of this study was to assess the role of balneal therapy on oxidative stressparameters in experimental myocardial ischemia induced in rats. 5 groups of 8 rats were used as follow:group 1- control group; group 2 - group swimming in distilled water (DW); group 3- group with myocardialischemia (MI); group 4 - group with MI swimming in DW; group 5 - group with MI and swimming incarbonated mineral water (CMW). Myocardial ischemia was induced with Isoproterenol. The followingoxidative stress/antioxidant blood parameters were assessed for each animal: nitric oxide (NOx),malondialdechyde (MDA), total oxidative stress (TOS), catalase (CAT) and total ant oxidative capacity ofplasma (TAC). In group 5 all parameters assessed were significantly improved compared with group 3 and4. Carbonated mineral water can be used as an adjuvant therapy for improving oxidative stress/antioxidantstatus in patients with cardiac ischemia, in order to reduce the amplitude of ischemic lesions and tocontribute as a prophylactic therapy to a better quality of life for these patients. Continuing this research inhumans through clinical studies would be warranted.
The main objective of this study was to analyze the perception of the influence of various weather conditions on patients with rheumatic pathology. A group of 394 patients, aged between 39 and 87 years and diagnosed with degenerative rheumatic diseases, were interviewed individually by using a questionnaire created specifically for this study. Further on, to assess the relationship between pain intensity and weather conditions, a frequency analysis based on Pearson’s correlation matrix was employed. The most important results are as follows: the great majority of the participants (more than 75%) believe that their rheumatic pain is definitely or to a great extent influenced by different weather conditions; most of the patients reported intensification of their pain with weather worsening, especially when cloudiness and humidity suddenly increase (83.8% and 82.0%, respectively), air temperature suddenly decreases (81.5%), and in fog or rain conditions (81.2%). In our research, alongside simple meteorological variables, we established that complex weather variables such as atmospheric fronts, in particular, the cold ones and winter anticyclonic conditions, greatly intensify the rheumatic pain, whereas summer anticyclonic conditions usually lead to a decrease in pain severity. In terms of relationships between pain intensity and weather conditions, we found the strongest correlations (ranging between 0.725 and 0.830) when temperature, relative humidity, and cloudiness are constantly high.
Epigallocatechin gallate (EGCG) has been intensively studied for its anti-oxidative, anti-inflammatory and anti-nociceptive effects. Our study aimed to assess the beneficial effect of liposomal EGCG (L-EGCG) co-administered with metoclopramide (MC) on oxidative stress and pain in experimental migraine induced by i.p. nitroglycerine (NG) administration in rats. Five groups of randomly divided rats (7/group) were investigated: control (C, group 1) with i.p. administration of saline solution, NG control group (group 2), NG+MC (group 3), NG+MC+EGCG group (group 4), and NG+MC+L-EGCG (group 5). The nociception was appreciated by the formalin test and the oxidative stress/anti-oxidant status by serum tests. MC alone significantly improved the nociception process and the oxidative stress parameters but not the antioxidative status. Adding EGCG to MC significantly reduced the oxidative stress and antioxidant status together with decreasing of nociception, with better results for L-EGCG.
Arylesterase activity of Paraoxonase 1 - prognostic factorArylesterase activity of Paraoxonase 1 - prognostic factorfor one-year survival in patients with acute myocardial infarction
Lorena Ciumărnean1, Mihai Greavu2, Ştefan C Vesa3, Alina I Tanțău1, Gabriela BDogaru4, Teodora G Alexescu1, Mircea V Milaciu1, George V Saraci5*, Antonia EMacarie6, Ioana Para1
Background: Curcumin is an antioxidant that reduces inflammation and pain. This study aimed to assess the effect of pretreatment with naproxen and liposomal curcumin compared with naproxen and curcumin solution on oxidative stress parameters and pain in a rat model of migraine. Material/Methods: Sixty-three male Wistar rats included a control group (n=9) and a rat model of migraine (n=54) induced by intraperitoneal injection of nitroglycerin (1 mg/0.1 kg). The rat model group was divided into an untreated control group (n=9), a group pretreated with naproxen alone (2.8 mg/kg) (n=9), a group pretreated with naproxen (2.8 mg/kg) combined with curcumin solution (1 mg/0.1 kg) (n=9), a group pretreated with naproxen (2.8 mg/kg) combined with curcumin solution (2 mg/0.1 kg) (n=9), a group pretreated with naproxen (2.8 mg/kg) combined with liposomal curcumin solution (1 mg/0.1 kg) (n=9) a group pretreated with naproxen (2.8 mg/kg) combined with liposomal curcumin solution (2 mg/0.1 kg) (n=9). Spectroscopy measured biomarkers of total oxidative status and nociception was tested using an injection of 1% of formalin into the rat paw. Results: Expression of biomarkers of oxidative stress and enhanced nociception were significantly increased following pretreatment with combined naproxen and liposomal curcumin compared with curcumin solution or naproxen alone (P<0.001). Combined curcumin solution and naproxen were more effective at a concentration of 2 mg/0.1kg for the first nociceptive phase (P<0.005). Conclusions: In a rat model of migraine, combined therapy with liposomal curcumin and naproxen showed an improved antioxidant effect and anti-nociceptive effect.
Adriana Elena Bulboacă 1,Alina S. Porfire 2,Lucia R. Tefas 2,Paul Mihai Boarescu 1,* ,Sorana D. Bolboacă 3,* OrcID,Ioana C. Stănescu 4,Angelo Corneliu Bulboacă 4 andGabriela Dogaru 5
Abstract: Curcumin (CC) is known to have anti-inflammatory and anti-oxidative properties and has already been tested for its efficiency in different diseases including diabetes mellitus (DM). New formulations and route administration were designed to obtain products with higher bioavailability. Our study aimed to test the effect of intraperitoneal (i.p.) administration of liposomal curcumin (lCC) as pre-treatment in streptozotocin(STZ)-induced DM in rats on oxidative stress, liver, and pancreatic functional parameters. Forty-two Wistar-Bratislava rats were randomly divided into six groups (seven animals/group): control (no diabetes), control-STZ (STZ-induced DM —60 mg/100g body weight a single dose intraperitoneal administration, and no CC pre-treatment), two groups with DM and CC pre-treatment (1mg/100g bw—STZ + CC1, 2 mg/100g bw—STZ + CC2), and two groups with DM and lCC pre-treatment (1 mg/100g bw—STZ + lCC1, 2 mg/100g bw—STZ + lCC1). Intraperitoneal administration of Curcumin in diabetic rats showed a significant reduction of nitric oxide, malondialdehyde, total oxidative stress, and catalase for both evaluated formulations (CC and lCC) compared to control group (p < 0.005), with higher efficacy of lCC formulation compared to CC solution (p < 0.002, excepting catalase for STZ + CC2vs. STZ + lCC1when p = 0.0845). The CC and lCC showed hepatoprotective and hypoglycemic effects, a decrease in oxidative stress and improvement in anti-oxidative capacity status against STZ-induced DM in rats (p < 0.002). The lCC also proved better efficacy on MMP-2, and -9 plasma levels as compared to CC (p < 0.003, excepting STZ + CC2 vs. STZ + lCC1 comparison with p = 0.0553). The lCC demonstrated significantly better efficacy as compared to curcumin solution on all serum levels of the investigated markers, sustaining its possible use as adjuvant therapy in DM.
Enhancement of bone consolidation using high-frequency pulse electromagnetic fiels (HF-PEMFs): An experimental study on rats
ADELA VIVIANA SITAR TAUT1,2,#, OLGA ORASAN1,2,#, ADRIANA FODOR1,3#, ANCA DANIELA FARCAS1,4*, SIMINA TARMURE SARLEA1,2, GABRIELA DOGARU1,5, DUMITRU TUDOR ZDRENGHEA1,6, DANA POP1,6, ANGELA COZMA 1,2,#
Were investigated the relationship between gender, cardiovascular risk factors and inflammation in metabolicsyndrome (MetS) patients. 100 consecutive patients (75 women), 73 with MetS, mean age 57.52±9.77years, were examined. Adhesion molecules (sICAM1, sVCAM1) were measured in the stored serum samplescollected using the ELISA method. The classification of MetS was based on IDF guidelines. The study wascarried out at the Department of Cardiology, Clinical Rehabilitation Hospital, Cluj-Napoca, Romania. MetSpatients presented lower sICAM1 values (225.01±86.75 ng/mL vs 234.22±82.23 ng/mL, p=NS), but highersVCAM1 values (605.34±298.69 ng/mL vs 552.29±233.77 ng/mL, p=NS). Differences between patientswith vs without metabolic syndrome were found only in men for sICAM1 (194.73±37.92 ng/mL vs 282±27.15ng/mL, p<0.001). Considering the HOMA index, a significant difference for sICAM1 was found in men(patients within the upper quartile vs the lower quartile, p=0.002), but also between women and menwithin the upper quartile of HOMA (for sICAM1 p=0.038). No significant differences were found for sVCAM1.In the case of males, sICAM1 was an independent predictor of metabolic syndrome, with a very goodcapacity to identify metabolic syndrome (AUROC=0.987, p=0.0001, Se=89.47%, Sp=100%). In conclusion,just in men, sICAM1 seems to have an excellent capacity to differentiate between MetS+ and MetS- patients,to predict MetS development.