Background: Curcumin is an antioxidant that reduces inflammation and pain. This study aimed to assess the effect of pretreatment with naproxen and liposomal curcumin compared with naproxen and curcumin solution on oxidative stress parameters and pain in a rat model of migraine. Material/Methods: Sixty-three male Wistar rats included a control group (n=9) and a rat model of migraine (n=54) induced by intraperitoneal injection of nitroglycerin (1 mg/0.1 kg). The rat model group was divided into an untreated control group (n=9), a group pretreated with naproxen alone (2.8 mg/kg) (n=9), a group pretreated with naproxen (2.8 mg/kg) combined with curcumin solution (1 mg/0.1 kg) (n=9), a group pretreated with naproxen (2.8 mg/kg) combined with curcumin solution (2 mg/0.1 kg) (n=9), a group pretreated with naproxen (2.8 mg/kg) combined with liposomal curcumin solution (1 mg/0.1 kg) (n=9) a group pretreated with naproxen (2.8 mg/kg) combined with liposomal curcumin solution (2 mg/0.1 kg) (n=9). Spectroscopy measured biomarkers of total oxidative status and nociception was tested using an injection of 1% of formalin into the rat paw. Results: Expression of biomarkers of oxidative stress and enhanced nociception were significantly increased following pretreatment with combined naproxen and liposomal curcumin compared with curcumin solution or naproxen alone (P<0.001). Combined curcumin solution and naproxen were more effective at a concentration of 2 mg/0.1kg for the first nociceptive phase (P<0.005). Conclusions: In a rat model of migraine, combined therapy with liposomal curcumin and naproxen showed an improved antioxidant effect and anti-nociceptive effect.

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Adriana Elena Bulboacă 1,Alina S. Porfire 2,Lucia R. Tefas 2,Paul Mihai Boarescu 1,* ,Sorana D. Bolboacă 3,* OrcID,Ioana C. Stănescu 4,Angelo Corneliu Bulboacă 4 andGabriela Dogaru 5

Abstract: Curcumin (CC) is known to have anti-inflammatory and anti-oxidative properties and has already been tested for its efficiency in different diseases including diabetes mellitus (DM). New formulations and route administration were designed to obtain products with higher bioavailability. Our study aimed to test the effect of intraperitoneal (i.p.) administration of liposomal curcumin (lCC) as pre-treatment in streptozotocin(STZ)-induced DM in rats on oxidative stress, liver, and pancreatic functional parameters. Forty-two Wistar-Bratislava rats were randomly divided into six groups (seven animals/group): control (no diabetes), control-STZ (STZ-induced DM —60 mg/100g body weight a single dose intraperitoneal administration, and no CC pre-treatment), two groups with DM and CC pre-treatment (1mg/100g bw—STZ + CC1, 2 mg/100g bw—STZ + CC2), and two groups with DM and lCC pre-treatment (1 mg/100g bw—STZ + lCC1, 2 mg/100g bw—STZ + lCC1). Intraperitoneal administration of Curcumin in diabetic rats showed a significant reduction of nitric oxide, malondialdehyde, total oxidative stress, and catalase for both evaluated formulations (CC and lCC) compared to control group (p < 0.005), with higher efficacy of lCC formulation compared to CC solution (p < 0.002, excepting catalase for STZ + CC2vs. STZ + lCC1when p = 0.0845). The CC and lCC showed hepatoprotective and hypoglycemic effects, a decrease in oxidative stress and improvement in anti-oxidative capacity status against STZ-induced DM in rats (p < 0.002). The lCC also proved better efficacy on MMP-2, and -9 plasma levels as compared to CC (p < 0.003, excepting STZ + CC2 vs. STZ + lCC1 comparison with p = 0.0553). The lCC demonstrated significantly better efficacy as compared to curcumin solution on all serum levels of the investigated markers, sustaining its possible use as adjuvant therapy in DM.


https://www.mdpi.com/1420-3049/24/5/846/htm

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ADELA VIVIANA SITAR TAUT1,2,#, OLGA ORASAN1,2,#, ADRIANA FODOR1,3#, ANCA DANIELA FARCAS1,4*, SIMINA TARMURE SARLEA1,2, GABRIELA DOGARU1,5, DUMITRU TUDOR ZDRENGHEA1,6, DANA POP1,6, ANGELA COZMA 1,2,#

Were investigated the relationship between gender, cardiovascular risk factors and inflammation in metabolicsyndrome (MetS) patients. 100 consecutive patients (75 women), 73 with MetS, mean age 57.52±9.77years, were examined. Adhesion molecules (sICAM1, sVCAM1) were measured in the stored serum samplescollected using the ELISA method. The classification of MetS was based on IDF guidelines. The study wascarried out at the Department of Cardiology, Clinical Rehabilitation Hospital, Cluj-Napoca, Romania. MetSpatients presented lower sICAM1 values (225.01±86.75 ng/mL vs 234.22±82.23 ng/mL, p=NS), but highersVCAM1 values (605.34±298.69 ng/mL vs 552.29±233.77 ng/mL, p=NS). Differences between patientswith vs without metabolic syndrome were found only in men for sICAM1 (194.73±37.92 ng/mL vs 282±27.15ng/mL, p<0.001). Considering the HOMA index, a significant difference for sICAM1 was found in men(patients within the upper quartile vs the lower quartile, p=0.002), but also between women and menwithin the upper quartile of HOMA (for sICAM1 p=0.038). No significant differences were found for sVCAM1.In the case of males, sICAM1 was an independent predictor of metabolic syndrome, with a very goodcapacity to identify metabolic syndrome (AUROC=0.987, p=0.0001, Se=89.47%, Sp=100%). In conclusion,just in men, sICAM1 seems to have an excellent capacity to differentiate between MetS+ and MetS- patients,to predict MetS development.

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Universitatea de Medicină și Farmacie „Iuliu Hațieganu” Cluj - Napoca

Proiect / studiu

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